Analytical Investigation of Forced Oxidized Anti-VEGF IgG Molecules: A Focus on the Alterations in Antigen and Receptor Binding Activities

Alterations in the biological activity of molecules under stress conditions have not been documented as widely literature yet. This study was designed to reveal functional impacts various oxidation on a model mAb, commercial anti-VEGF IgG molecule. The responses antigen binding, cell proliferation, FcRn receptors, and C1q which rarely appear current literature, were investigated. authors report peptide mapping data, post-translational modification (PTM) analysis, proliferation performance, (VEGF), C1q, binding activities mAb conditions. oxidation-prone site determined Met252 DTLMISR peptide. VEGF anti-cell mAbs did alter, while capacity significantly decreased oxidative full is vital for many scientific industrial processes about mAbs. recommend performing analyses addition structural studies investigating factors therapeutic